Antioxidant and anti-inflammatory activities of Oroxylum indicum Kurz (L.) fruit extract in lipopolysaccharide-stimulated BV2 microglial cells


 Purpose: To investigate the anti-inflammatory effects and antioxidant activity of Oroxylum indicum (L.) Kurz fruit extract in lipopolysaccharide (LPS)-stimulated BV2 microglia.
 Methods: cells were treated with LPS for 24 h presence or absence O. extract. Then, nitric oxide (NO), reactive oxygen species (ROS) interleukin 6 (IL-16) levels measured using Griess reagent assay, CM-H2DCFDA enzyme-linked immunosorbent (ELISA) assays, respectively. The vitro property was also investigated by 1,1- diphenyl-2-picrylhydrazyl (DPPH) 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assays.
 Results: Levels IL-6, NO, ROS LPS-treated significantly higher than those control (p < 0.01). However, exposure to led a marked decrease these parameters, when compared untreated Results from DPPH ABTS assays showed that exhibited good properties, total flavonoid phenolic contents 115.58 ± 1.09 131.04 2.37 mg/g dried extract, respectively.
 Conclusion: results demonstrate exerts anti-oxidant LPS-stimulated cells. Thus might be beneficial development novel anti-oxidative anti-neuroinflammatory herbal medicines. mechanisms which indium fruits reduces NO IL-6 needs further investigated.